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 | Acceso al texto completo restringido a Biblioteca INIA Las Brujas. Por información adicional contacte bibliolb@inia.org.uy. |
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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
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Fecha : |
22/07/2018 |
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Actualizado : |
22/07/2018 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Autor : |
FERNÁNDEZ, S.; FRAGA, M.; SILVEYRA, E.; TROMBERT, A.N.; RABAZA, A.; PLA, M. |
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Afiliación : |
S. FERNÁNDEZ, MEC/ IIBCE (Instituto de Investigaciones de Ciencias Biológicas "Clemente Estable"); MARTIN FRAGA COTELO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; E. SILVEYRA, MEC/ IIBCE (Instituto de Investigaciones de Ciencias Biológicas "Clemente Estable"); A.N. TROMBERT, Universidad Mayor, Santiago (Chile); A. RABAZA, MEC/ IIBCE (Instituto de Investigaciones de Ciencias Biológicas "Clemente Estable"); MARCELO PLA TEJERA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. |
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Título : |
Probiotic properties of native Lactobacillus spp. strains for dairy calves. |
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Complemento del título : |
Research article. |
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Fecha de publicación : |
2018 |
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Fuente / Imprenta : |
Beneficial Microbes, April 2018, vol.9, no.4, p. 613 - 624. |
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ISSN : |
1876-2883 |
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DOI : |
10.3920/BM2017.0131 |
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Idioma : |
Inglés |
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Notas : |
Artilce history: Received: 12 September 2017 / Accepted: 22 December 2017 / Published Online: April 10, 2018. |
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Contenido : |
ABSTRACT.
The use of native microorganisms with probiotic capacity is an alternative tool for the treatment and prevention of several diseases that affect animals, such as neonatal calf diarrhoea. The selection of probiotic strains within a collection is based on different in vitro and in vivo assays, which predict their potential. The aim of this study was to characterise a group of native Lactobacillus spp. strains isolated from faeces of healthy calves using an in vitro approach and to assess their ability to colonise the gastrointestinal tract (GIT) of calves. Native Lactobacillus spp. strains were evaluated on their capacity to survive low pH conditions and bile salts presence, biofilm formation and adhesion to both mucus and Caco-2 cells. Based on the in vitro characterisation, four strains (Lactobacillus johnsonii TP1.1, Lactobacillus reuteri TP1.3B, L. johnsonii TP1.6 and Lactobacillus amylovorus TP8.7) were selected to evaluate their capacity to colonise and persist in the GIT of calves. The assessment of enteric persistence involved an in vivo assay with oral administration of probiotics and quantification in faeces of the administered bacterial species with real-time quantitative PCR (qPCR). The study was conducted using 15 calves (1-month-old) which were divided into five groups of three animals, four of which were treated with four different selected strains and one was the control group. Strains TP1.3B and TP1.6 managed to persist in treated animals until ten days after the end of the administration period, indicating that they could be promising candidates for the design of probiotics for calves.
© 2018 Wageningen Academic Publishers. MenosABSTRACT.
The use of native microorganisms with probiotic capacity is an alternative tool for the treatment and prevention of several diseases that affect animals, such as neonatal calf diarrhoea. The selection of probiotic strains within a collection is based on different in vitro and in vivo assays, which predict their potential. The aim of this study was to characterise a group of native Lactobacillus spp. strains isolated from faeces of healthy calves using an in vitro approach and to assess their ability to colonise the gastrointestinal tract (GIT) of calves. Native Lactobacillus spp. strains were evaluated on their capacity to survive low pH conditions and bile salts presence, biofilm formation and adhesion to both mucus and Caco-2 cells. Based on the in vitro characterisation, four strains (Lactobacillus johnsonii TP1.1, Lactobacillus reuteri TP1.3B, L. johnsonii TP1.6 and Lactobacillus amylovorus TP8.7) were selected to evaluate their capacity to colonise and persist in the GIT of calves. The assessment of enteric persistence involved an in vivo assay with oral administration of probiotics and quantification in faeces of the administered bacterial species with real-time quantitative PCR (qPCR). The study was conducted using 15 calves (1-month-old) which were divided into five groups of three animals, four of which were treated with four different selected strains and one was the control group. Strains TP1.3B and TP1.6 managed to persist in treated animals until ten d... Presentar Todo |
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Palabras claves : |
GASTROINTESTINAL TRACT; LACTOBACILLUS SPP; NATIVE; NEONATAL CALF DIARRHOEA; PROBIOTIC POTENTIAL. |
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Thesagro : |
GANADO DE LECHE. |
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Asunto categoría : |
-- |
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Marc : |
LEADER 02595naa a2200289 a 4500
001 1058830
005 2018-07-22
008 2018 bl uuuu u00u1 u #d
022 $a1876-2883
024 7 $a10.3920/BM2017.0131$2DOI
100 1 $aFERNÁNDEZ, S.
245 $aProbiotic properties of native Lactobacillus spp. strains for dairy calves.$h[electronic resource]
260 $c2018
500 $aArtilce history: Received: 12 September 2017 / Accepted: 22 December 2017 / Published Online: April 10, 2018.
520 $aABSTRACT. The use of native microorganisms with probiotic capacity is an alternative tool for the treatment and prevention of several diseases that affect animals, such as neonatal calf diarrhoea. The selection of probiotic strains within a collection is based on different in vitro and in vivo assays, which predict their potential. The aim of this study was to characterise a group of native Lactobacillus spp. strains isolated from faeces of healthy calves using an in vitro approach and to assess their ability to colonise the gastrointestinal tract (GIT) of calves. Native Lactobacillus spp. strains were evaluated on their capacity to survive low pH conditions and bile salts presence, biofilm formation and adhesion to both mucus and Caco-2 cells. Based on the in vitro characterisation, four strains (Lactobacillus johnsonii TP1.1, Lactobacillus reuteri TP1.3B, L. johnsonii TP1.6 and Lactobacillus amylovorus TP8.7) were selected to evaluate their capacity to colonise and persist in the GIT of calves. The assessment of enteric persistence involved an in vivo assay with oral administration of probiotics and quantification in faeces of the administered bacterial species with real-time quantitative PCR (qPCR). The study was conducted using 15 calves (1-month-old) which were divided into five groups of three animals, four of which were treated with four different selected strains and one was the control group. Strains TP1.3B and TP1.6 managed to persist in treated animals until ten days after the end of the administration period, indicating that they could be promising candidates for the design of probiotics for calves. © 2018 Wageningen Academic Publishers.
650 $aGANADO DE LECHE
653 $aGASTROINTESTINAL TRACT
653 $aLACTOBACILLUS SPP
653 $aNATIVE
653 $aNEONATAL CALF DIARRHOEA
653 $aPROBIOTIC POTENTIAL
700 1 $aFRAGA, M.
700 1 $aSILVEYRA, E.
700 1 $aTROMBERT, A.N.
700 1 $aRABAZA, A.
700 1 $aPLA, M.
773 $tBeneficial Microbes, April 2018, vol.9, no.4, p. 613 - 624.
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Registro original : |
INIA Las Brujas (LB) |
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Registro completo
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Biblioteca (s) : |
INIA Tacuarembó. |
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Fecha actual : |
21/12/2017 |
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Actualizado : |
02/03/2018 |
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Tipo de producción científica : |
Artículos en Revistas Agropecuarias |
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Autor : |
BLANCO, P.H.; MOLINA, F.; PÉREZ DE VIDA, F.; MARTÍNEZ, S.; CARRACELAS, G.; PEREIRA, A.L.; CASTILLO, J.; SALDAIN, N.E.; VARGAS, J.; VILLALBA, M.; ESCALANTE, F.; FERREIRA, A.; SOSA, B. |
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Afiliación : |
PEDRO HORACIO BLANCO BARRAL, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; FEDERICO MOLINA CASELLA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; FERNANDO BLAS PEREZ DE VIDA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; SEBASTIÁN MARTÍNEZ KOPP, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; JULIO GONZALO CARRACELAS GARRIDO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; ANA LAURA PEREIRA AMATO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; EMILSE JESUS CASTILLO VELAZQUEZ, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; NÉSTOR ELIO SALDAIN CROCCE, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; JOSÉ VARGAS; MARIO VILLALBA; FERNANDO ESCALANTE; ALEXANDRA FERREIRA; BETO SOSA. |
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Título : |
Nuevos cultivares de arroz para el sistema CLEARFIELD® INIA CL212 E INIA CL244. |
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Fecha de publicación : |
2017 |
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Fuente / Imprenta : |
Revista INIA Uruguay, 2017, no.51, p.20-24. |
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Serie : |
(Revista INIA; 51) |
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ISSN : |
1510-9011 |
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Idioma : |
Español |
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Contenido : |
os cultivares Clearfield® INIA CL212 e INIA CL244 se han cultivado en los últimos años bajo el sistema de validación, acordado con el Consorcio Nacional de Semilleristas
de Arroz (CNSA). En la zafra 2016/17 alcanzaron un área cultivada de 6453 y 1108 ha, respectivamente, representando conjuntamente el 4,5% del área total de
cultivo y 20% de la superficie cultivada con el Sistema Clearfield®. Estos cultivares fueron desarrollados en el marco de un acuerdo de investigación con BASF, contando
con la segunda generación de resistencia a imidazolinonas desarrollada por Louisiana State University (LSU). Recientemente, INIA obtuvo una sub-licencia de esta tecnología, lo que posibilita la comercialización de estos cultivares, que se encuentran en proceso de licenciamiento al CNSA. |
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Palabras claves : |
RICE (ORYZA SATIVA L.). |
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Thesagro : |
ARROZ. |
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Asunto categoría : |
F01 Cultivo |
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URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/8185/1/revista-INIA-51-diciembre-2017.-p.20-24.pdf
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Marc : |
LEADER 01613naa a2200313 a 4500
001 1057912
005 2018-03-02
008 2017 bl uuuu u00u1 u #d
022 $a1510-9011
100 1 $aBLANCO, P.H.
245 $aNuevos cultivares de arroz para el sistema CLEARFIELD® INIA CL212 E INIA CL244.
260 $c2017
490 $a(Revista INIA; 51)
520 $aos cultivares Clearfield® INIA CL212 e INIA CL244 se han cultivado en los últimos años bajo el sistema de validación, acordado con el Consorcio Nacional de Semilleristas de Arroz (CNSA). En la zafra 2016/17 alcanzaron un área cultivada de 6453 y 1108 ha, respectivamente, representando conjuntamente el 4,5% del área total de cultivo y 20% de la superficie cultivada con el Sistema Clearfield®. Estos cultivares fueron desarrollados en el marco de un acuerdo de investigación con BASF, contando con la segunda generación de resistencia a imidazolinonas desarrollada por Louisiana State University (LSU). Recientemente, INIA obtuvo una sub-licencia de esta tecnología, lo que posibilita la comercialización de estos cultivares, que se encuentran en proceso de licenciamiento al CNSA.
650 $aARROZ
653 $aRICE (ORYZA SATIVA L.)
700 1 $aMOLINA, F.
700 1 $aPÉREZ DE VIDA, F.
700 1 $aMARTÍNEZ, S.
700 1 $aCARRACELAS, G.
700 1 $aPEREIRA, A.L.
700 1 $aCASTILLO, J.
700 1 $aSALDAIN, N.E.
700 1 $aVARGAS, J.
700 1 $aVILLALBA, M.
700 1 $aESCALANTE, F.
700 1 $aFERREIRA, A.
700 1 $aSOSA, B.
773 $tRevista INIA Uruguay, 2017, no.51, p.20-24.
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INIA Tacuarembó (TBO) |
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